Molecularly imprinted solid-phase extraction of cephalexin from water-based matrices.
J Sep Sci
; 32(19): 3319-26, 2009 Oct.
Article
en En
| MEDLINE
| ID: mdl-19722176
ABSTRACT
In the present paper, we describe the synthesis of a cephalexin (CFX) molecularly imprinted polymer (MIP), the direct application of the MIP to SPE for the determination of CFX (which is a beta-lactam antibiotic) in human urine and the use of the MIP in a tandem SPE system to determine CFX in river water. The molecularly imprinted polymers (MIP) showed cross-selectivity for amoxicillin (AMX; also a beta-lactam antibiotic). This allowed both CFX and AMX to be quantified in acidified human urine, with recoveries of 78 and 60% for CFX and AMX, respectively, when the urine samples were spiked with CFX and AMX at 4 mg/L. These analyses were facile because the molecularly imprinted solid-phase extraction (MISPE) extracts were clear compared to the nonpurified samples. In order to increase the sample volume for river water analyses, a tandem SPE system incorporating a commercially available sorbent was implemented. With this set-up, CFX was determined with recoveries in excess of 50% when 200 mL of acidified river water samples spiked at 10 microg/L with CFX were percolated through the tandem system.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Polímeros
/
Agua
/
Cefalexina
/
Extracción en Fase Sólida
/
Impresión Molecular
/
Antibacterianos
Tipo de estudio:
Evaluation_studies
Límite:
Humans
Idioma:
En
Año:
2009
Tipo del documento:
Article