Optimization of the enzymatic production of 20(S)-ginsenoside Rg(3) from white ginseng extract using response surface methodology.
N Biotechnol
; 26(3-4): 181-6, 2009 Oct 31.
Article
en En
| MEDLINE
| ID: mdl-19735748
ABSTRACT
The optimization of the conversion of ginseng saponin glycosides to 20(S)-ginsenoside Rg(3) by enzymatic transformation was carried out using response surface methodology (RSM) based on a 2(3) factorial central composite design. The production of 20(S)-ginsenoside Rg(3) using several commercial enzymes indicated that the enzyme Cellulase-12T was the most efficient at producing 20(S)-ginsenoside Rg(3). To optimize the enzymatic production of 20(S)-ginsenoside Rg(3), response surface methodology was applied to determine the ideal amount of white ginseng extract, Cellulase-12T and reaction time. These results indicate that white ginseng extract (1.67%) treated with Celluase-12T (3.67%) for 72 hours had 4 times the quantity of 20(S)-ginsenoside Rg(3) compared to commercial white ginseng extract.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Biotecnología
/
Extractos Vegetales
/
Celulasa
/
Ginsenósidos
/
Panax
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Año:
2009
Tipo del documento:
Article