Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS.
J Sep Sci
; 32(21): 3579-84, 2009 Nov.
Article
en En
| MEDLINE
| ID: mdl-19813228
ABSTRACT
The nutritional value of maize seed is limited due to its high content of storage proteins (zeins), which are deficient in essential amino acids such as lysine and tryptophan. In a previous paper, we showed that protein bodies obtained from BR473 maize variety, developed by Embrapa (Brazilian Agricultural Research Corporation), were mainly constituted by Z27 and a smaller quantity of Z50 gamma-zeins. Besides zein proteins, other not identified protein band in the SDS/PAGE was also observed, which could indicate the presence of non-zein proteins additionally to gamma-zeins. In the present paper, we have demonstrated the presence of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS and database searching. This fact could be related to the excellent energetic value and higher protein quality of BR473 maize grains, since high lysine concentration in some maize varieties has been related to the presence of cytoskeleton proteins that are non-zeins. We have identified the following proteins Brittle-1 protein (chloroplast precursor), Legumin-1, glyceroldehyde-3-phosphate dehydrogenase, and elongation factor 1-alpha.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Proteínas de Plantas
/
Zeína
/
Cromatografía Liquida
/
Zea mays
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Espectrometría de Masa por Ionización de Electrospray
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Espectrometría de Masas en Tándem
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Año:
2009
Tipo del documento:
Article