Diffusion and binding of monoclonal antibody TNT-1 in multicellular tumor spheroids.
J Natl Cancer Inst
; 83(3): 200-4, 1991 Feb 06.
Article
en En
| MEDLINE
| ID: mdl-1988705
ABSTRACT
Tumor spheroids of HT-29 human colon adenocarcinoma and A375 melanoma were established to investigate the uptake and clearance kinetics of TNT-1, a monoclonal antibody that targets necrotic cells of tumors. Our data reveal that there was rapid uptake of TNT-1 and its F(ab')2 fragment in both spheroid models, whereas an antibody of irrelevant specificity, Lym-1, and its F(ab')2 fragment bound poorly to the spheroids. Unlike previously reported monoclonal antibodies to tumor cell-surface antigens, TNT-1 showed 1) a linear uptake that increased over time without saturation in tumor spheroids and 2) an unexpected uptake by a subpopulation of cells in the viable outer rim of the spheroids. These preclinical studies provide important information concerning the therapeutic potential of TNT monoclonal antibodies for the treatment of cancer and micrometastases.
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Banco de datos:
MEDLINE
Asunto principal:
Células Tumorales Cultivadas
/
Anticuerpos Monoclonales
Límite:
Humans
Idioma:
En
Año:
1991
Tipo del documento:
Article