Cloning, expression and characterization of Mycobacterium tuberculosis lipoprotein LprF.
Biochem Biophys Res Commun
; 391(1): 679-84, 2010 Jan 01.
Article
en En
| MEDLINE
| ID: mdl-19944079
ABSTRACT
Lipoproteins are well known virulence factors of bacterial pathogens in general and of Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, in particular. Lipoprotein lipidation between Gram-positive and Gram-negative bacteria differs significantly as these are di- and triacylated, respectively. Little is known about the lipid anchor of mycobacterial lipoproteins. We reported recently that mycobacterial LppX, a lipoprotein involved in synthesis of cell wall components is triacylated, although mycobacteria are classified as GC-rich Gram-positive bacteria. We here exploited the model organism Mycobacterium smegmatis for the expression of Mtb LprF and characterized N-terminal modifications at the molecular level. LprF is a putative lipoprotein of Mtb involved in signaling of potassium-dependent osmotic stress. LprF is extensively modified in a mycobacterium-specific manner by a thioether-linked diacylglyceryl residue with one ester-bound tuberculostearic- and one C160 fatty acid and additionally by a third N-linked C160 fatty acid, and a hexose.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Lipoproteínas
/
Mycobacterium tuberculosis
Tipo de estudio:
Prognostic_studies
Idioma:
En
Año:
2010
Tipo del documento:
Article