L-4F alters hyperlipidemic (but not healthy) mouse plasma to reduce platelet aggregation.
Arterioscler Thromb Vasc Biol
; 30(2): 283-9, 2010 Feb.
Article
en En
| MEDLINE
| ID: mdl-19965777
ABSTRACT
UNLABELLED Background and Purpose- Hyperlipidemia is associated with platelet hyperreactivity. We hypothesized that L-4F, an apolipoprotein A-I mimetic peptide, would inhibit platelet aggregation in hyperlipidemic mice. METHODS AND RESULTS:
Injecting L-4F into apolipoprotein E (apoE)-null and low-density lipoprotein receptor-null mice resulted in a significant reduction in platelet aggregation in response to agonists; however, there was no reduction in platelet aggregation after injection of L-4F into wild-type (WT) mice. Consistent with these results, injection of L-4F into apoE-null mice prolonged bleeding time; the same result was not found in WT mice. Incubating L-4F in vitro with apoE-null platelet-rich plasma also resulted in decreased platelet aggregation. However, incubating washed platelets from either apoE-null or WT mice with L-4F did not alter aggregation. Compared with WT mice, unstimulated platelets from apoE-null mice contained significantly more 12-hydroxy 5,8,10,14-eicosatetraenoic acid, thromboxane A(2), and prostaglandins D(2) and E(2). In response to agonists, platelets from L-4F-treated apoE-null mice formed significantly less thromboxane A(2), prostaglandins D(2) and E(2), and 12-hydroxy 5,8,10,14-eicosatetraenoic acid.CONCLUSIONS:
By binding plasma-oxidized lipids that cause platelet hyperreactivity in hyperlipidemic mice, L-4F decreases platelet aggregation.
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1
Banco de datos:
MEDLINE
Asunto principal:
Péptidos
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Inhibidores de Agregación Plaquetaria
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Agregación Plaquetaria
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Apolipoproteína A-I
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Hiperlipidemias
Límite:
Animals
Idioma:
En
Año:
2010
Tipo del documento:
Article