Crevicular fluid glutathione levels in periodontitis and the effect of non-surgical therapy.
J Clin Periodontol
; 37(1): 17-23, 2010 Jan.
Article
en En
| MEDLINE
| ID: mdl-19968740
ABSTRACT
AIM:
To quantify reduced and oxidized glutathione (GSH and GSSG) levels in gingival crevicular fluid (GCF) of periodontitis patients pre-therapy (versus periodontally healthy controls) and ascertain whether successful non-surgical therapy alters glutathione levels. MATERIALS ANDMETHODS:
Thirty-second GCF samples (6/subject) were collected on Periopaper() strips from starved, non-smokers (n=20; mean age 43.6 years) with chronic periodontitis, before and 3 months after non-surgical therapy, and periodontally healthy, age- and gender-matched controls (n=20). GSH and GSSG levels were determined using reversed-phase high-performance liquid chromatography with fluorescence detection.RESULTS:
Lower concentrations of GSH (p<0.01) and GSSG (p<0.05) were detected in GCF from patients (pre- and post-therapy) than controls and treatment had no significant effect. Amounts per 30-second sample did not differ between patients and controls. However, the amount of GSSG per 30-second sample decreased in patients after therapy (p<0.05). Consequently, therapy increased the GSHGSSG ratio (p<0.05) in patients compared with the controls (p=0.8).CONCLUSION:
These data demonstrate high concentrations of GSH within GCF, which are compromised in chronic periodontitis. While therapy does not appear to fully restore GSH concentrations in GCF, it does restore the redox balance (GSHGSSG ratio), suggesting that the abnormal redox balance arises secondary to oxidative stress resulting from periodontal inflammation.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Líquido del Surco Gingival
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Periodontitis Crónica
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Glutatión
Tipo de estudio:
Observational_studies
/
Prognostic_studies
Límite:
Adult
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Female
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Humans
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Male
Idioma:
En
Año:
2010
Tipo del documento:
Article