Expression of functional GPR35 in human iNKT cells.
Biochem Biophys Res Commun
; 398(3): 420-5, 2010 Jul 30.
Article
en En
| MEDLINE
| ID: mdl-20599711
ABSTRACT
The aim of this study was to examine the expression of G protein-coupled receptor (GPR)35 in human invariant natural killer T (iNKT) cells and to determine the functional effects induced by selective activation of this receptor. RT-PCR analysis showed that both human iNKT cells and resting PBMC expressed GPR35; GPR35 protein resulted mostly localized in the plasma membrane, while it internalized in punctate intracellular structures following specific receptor activation (Western blot and immunofluorescence/confocal microscopy analysis). The specific activation of GPR35 by selective receptor agonists [l-kynurenic acid (KYNA)] or 1,4-dihydro-5-(2-propoxyphenyl)-7H-1,2,3-triazolo [4,5-d]pyrimidine-7-one (zaprinast)] functionally correlated with a significant reduction in IL-4 release from alpha-galactosylceramide (alpha-GalCer)-activated human iNKT cells, and this effect resulted mediated by pertussis toxin (PTX)-sensitive Gi/o proteins. In conclusion, our results demonstrate that human iNKT cells express GPR35 functionally active in reducing IL-4 release.
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Banco de datos:
MEDLINE
Asunto principal:
Membrana Celular
/
Receptores Acoplados a Proteínas G
/
Células T Asesinas Naturales
Límite:
Humans
Idioma:
En
Año:
2010
Tipo del documento:
Article