Reduced placental prolyl hydroxylase 3 mRNA expression in pregnancies affected by fetal growth restriction.
BJOG
; 117(13): 1635-42, 2010 Dec.
Article
en En
| MEDLINE
| ID: mdl-21040392
ABSTRACT
OBJECTIVE:
To investigate the role of the hypoxia-inducible factor (HIF) pathway in fetal growth restriction (FGR).DESIGN:
A case-control study.SETTING:
Research laboratory and gynaecology clinic. SAMPLE Twenty placentas from normal pregnancies and 20 from FGR pregnancies.METHODS:
RNA extraction, cDNA synthesis, quantitative real-time polymerase chain reaction (qRT-PCR) assay, statistical analysis. MAIN OUTCOMEMEASURES:
mRNA expression of HIF-1α, HIF-2α and HIF-ß (ARNT), along with prolyl hydroxylase domain 3 (PHD3), which leads to proteasomal degradation of HIF-α subunits.RESULTS:
No statistically significant differences in the transcription levels of ARNT and HIF-2α were found between FGR and normal placentas. By contrast, PHD3 and HIF-1α mRNA were downregulated in FGR placentas. PHD3 mRNA expression was associated with gestational age at delivery (P = 0.008), birthweight centile (P = 0.029) and abnormal umbilical artery (UA) Doppler measurements (P = 0.034).CONCLUSIONS:
As PHD3 regulates the HIF-mediated hypoxic response in FGR, we deduce that fetal adaptation to hypoxia ranges from impaired to adequate, as observed by the gradient of PHD3 downregulation in relation to the severity of FGR.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
ARN Mensajero
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Procolágeno-Prolina Dioxigenasa
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Retardo del Crecimiento Fetal
Tipo de estudio:
Observational_studies
/
Risk_factors_studies
Límite:
Adult
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Female
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Humans
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Male
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Pregnancy
Idioma:
En
Año:
2010
Tipo del documento:
Article