Evaluation of an immunomagnetic separation assay in combination with cultivation to improve Legionella pneumophila serogroup 1 recovery from environmental samples.
J Appl Microbiol
; 110(4): 952-61, 2011 Apr.
Article
en En
| MEDLINE
| ID: mdl-21276145
ABSTRACT
AIMS:
Legionella isolation from environmental samples is often difficult because of the presence of heterotrophic-associated bacteria that frequently overgrow when using standard culture (ISO 11731, 1998; NF T90-431, 2003) methods. To improve Legionella pneumophila recovery from complex water samples (water from cooling towers, biofilms), we evaluated an immunomagnetic separation (IMS) assay using a monoclonal antibody raised against the lipopolysaccharide of Leg. pneumophila sg1 in combination with culture. METHODS ANDRESULTS:
This study was conducted on 51 environmental specimens. The comparison between IMS-culture and standard culture (ISO 11731, 1998; NF T90-431, 2003) methods was made using ISO 17994, 2004 criteria for establishing equivalence between microbiological methods based on the upper and lower (XH and XL) values of the relative difference (95% confidence limit) and D as maximum acceptable deviation (value of the confidence limit).CONCLUSIONS:
We found that the average performance of IMS culture was higher than the reference method.
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Banco de datos:
MEDLINE
Asunto principal:
Legionella pneumophila
/
Separación Inmunomagnética
/
Microbiología Ambiental
Tipo de estudio:
Evaluation_studies
Idioma:
En
Año:
2011
Tipo del documento:
Article