In vitro requirement for periostin in B lymphopoiesis.
Blood
; 117(14): 3770-9, 2011 Apr 07.
Article
en En
| MEDLINE
| ID: mdl-21285437
ABSTRACT
B lymphopoiesis arrests in rabbits by 4 months of age. To identify molecules that contribute to this arrest, cDNA-representational difference analysis on BM stromal cells from young and adult rabbits showed that expression of Postn that encodes for the extracellular matrix protein periostin dramatically reduced with age. Postn-small interfering RNA OP9 cells lost their capacity to support B-cell development from rabbit or murine BM cells, and reexpression of periostin restored this potential, indicating an in vitro requirement for periostin in B lymphopoiesis. In our system, we determined that periostin deficiency leads to increased cell death and decreased proliferation of B-lineage progenitors. Further, RGD peptide inhibition of periostin/α(v)ß(3) interaction resulted in a marked decrease in B lymphopoiesis in vitro. Microarray analysis of the Postn-small interfering RNA OP9 cells showed decreased expression of key B-lymphopoietic factors, including IL-7 and CXCL12. In vivo, unidentified molecule(s) probably compensate periostin loss because Postn(-/-) mice had normal numbers of B-cell progenitors in BM. We conclude that the decline in periostin expression in adult rabbit BM does not solely explain the arrest of B lymphopoiesis. However, the interaction of periostin with α(v)ß(3) on lymphoid progenitors probably provides both proliferative and survival signals for cells in the B-cell development pathway.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Linfocitos B
/
Moléculas de Adhesión Celular
/
Linfopoyesis
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Año:
2011
Tipo del documento:
Article