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[Genetic polymorphisms of nine non-DNA combined index system short tandem repeat loci in Hebei Han population and application in paternity testing].
Guan, Ya-qing; Fu, Li-hong; Zhang, Xiao-jing; Li, Shu-jin; Cong, Bin; Ma, Chun-ling.
Afiliación
  • Guan YQ; Hebei Key Laboratory of Forensic Medicine, Department of Forensic Medicine, Hebei Medical University, Shijiazhuang, Hebei, 050017 PR China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 28(1): 103-7, 2011 Feb.
Article en Zh | MEDLINE | ID: mdl-21287521
ABSTRACT

OBJECTIVE:

To investigate the polymorphisms of 9 non-DNA combined index system (CODIS) short tandem repeats (STRs), i.e., D7S3048, D8S1132, D11S2368, D2S1772, D6S1043, D13S325, D12S391, GATA198B05, D18S1364 in Hebei Han population, and evaluate the usage of them in paternity testing.

METHODS:

One hundred and forty-seven unrelated healthy individuals from the Han population of Hebei province were genotyped using STRtyper10G kit including 9 STR loci on ABI 3130 Genetic Analyzer. Hardy-Weinberg equilibrium and population genetic parameters were calculated. Fourteen cases of motherless paternity testing and 2 cases of standard trios with mutation in 1 locus were detected using STRtyper10G.

RESULTS:

(1) Ninety-nine alleles and 336 genotypes were observed in the 9 STR loci in the population. The cumulative discrimination power(DP) was higher than 0.999,999,999. The cumulative probability of exclusion (PE) for trios and duos were 0.999,974 and 0.998,759 respectively. Departure from Hardy-Weinberg equilibrium was not observed in any of the 9 loci. (2) The combined paternity index (PI) of the 14 cases of motherless paternity testing ranged from 10³-106 for 15 STR loci in ID, whereas it reached 105-109 for 22 independent STR loci included in ID and STRtyper 10G. Possible mutation in FGA and vWA was observed in 2 cases of trios, and the combined PI was 5945 and 1840 respectively for 15 STR loci in ID. Adding STRtyper 10G to detect these 2 cases, the combined PI reached 2.76 × 107 and 4.88 × 107 respectively.

CONCLUSION:

The genetic polymorphism of the 9 non-CODIS STR loci included in STRtyper 10G was quite high in Chinese Hebei Han population, indicating the 9 STR loci are valuable as complement markers for ID and PP16 kit in motherless paternity testing, paternity testing with mutation and other kinds of complicated paternity testing.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Paternidad / Polimorfismo Genético / Repeticiones de Microsatélite Límite: Humans País/Región como asunto: Asia Idioma: Zh Año: 2011 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Paternidad / Polimorfismo Genético / Repeticiones de Microsatélite Límite: Humans País/Región como asunto: Asia Idioma: Zh Año: 2011 Tipo del documento: Article