Engineering of Escherichia coli for targeted delivery of transgenes to HER2/neu-positive tumor cells.
Biotechnol Bioeng
; 108(7): 1662-72, 2011 Jul.
Article
en En
| MEDLINE
| ID: mdl-21337329
ABSTRACT
Targeting of non-phagocytic tumor cells and prompt release of gene cargos upon entry into tumors are two limiting steps in the bacterial gene delivery path. To tackle these problems, the non-pathogenic Escherichia coli strain BL21(DE3) was engineered to display the anti-HER2/neu affibody on the surface. After co-incubation with tumor cells for 3 h, the anti-HER2/neu affibody-presenting E. coli strain was selectively internalized into HER2/neu-positive SKBR-3 cells. The invasion efficiency reached as high as 30%. Furthermore, the bacteria were equipped with the phage ÏX174 lysin gene E-mediated autolysis system. Carrying the transgene (e.g., eukaryotic green fluorescent protein, GFP), the tumor-targeting bacteria were subjected to the thermal shock to trigger the autolysis system upon entry into HER2/neu-positive cells. Flow cytometric analysis revealed that 3% of infected cells expressed GFP 24 h post thermal induction. Overall, the results show a promise of the proposed approach for developing bacteria as a delivery carrier.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Técnicas de Transferencia de Gen
/
Transgenes
/
Transferencia de Gen Horizontal
/
Escherichia coli
Límite:
Humans
Idioma:
En
Año:
2011
Tipo del documento:
Article