Centrosome duplication continues in cycloheximide-treated Xenopus blastulae in the absence of a detectable cell cycle.
J Cell Biol
; 110(6): 2033-42, 1990 Jun.
Article
en En
| MEDLINE
| ID: mdl-2190990
ABSTRACT
Cycloheximide (500 micrograms/ml) rapidly arrests cleavage, spindle assembly, and cycles of an M-phase-specific histone kinase in early Xenopus blastulae. 2 h after cycloheximide addition, most cells contained two microtubule asters radiating from perinuclear microtubule organizing centers (MTOCs). In contrast, blastomeres treated with cycloheximide for longer periods (3-6 h) contained numerous microtubule asters and MTOCs. Immunofluorescence with an anticentrosome serum and EM demonstrated that the MTOCs in cycloheximide-treated cells were typical centrosomes, containing centrioles and pericentriolar material. We conclude that centrosome duplication continues in cycloheximide-treated Xenopus blastulae in the absence of a detectable cell cycle. In addition, these observations suggest that Xenopus embryos contain sufficient material to assemble 1,000-2,000 centrosomes in the absence of normal protein synthesis.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Xenopus laevis
/
Blastocisto
/
Centriolos
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Cicloheximida
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Huso Acromático
Límite:
Animals
Idioma:
En
Año:
1990
Tipo del documento:
Article