Three-dimensional cultures of human endometrial cells on Matrigel mimic in vivo morphology.
Chin Med J (Engl)
; 125(5): 863-8, 2012 Mar.
Article
en En
| MEDLINE
| ID: mdl-22490588
ABSTRACT
BACKGROUND:
The regulation of endometrial physiology and morphogenesis by the paracrine effectors has been well established using in vivo studies. A more complete understanding of the endometrial function has been delayed due, in part, to a lack of appropriate culture models. In this study, we aimed to simulate the in vivo three-dimensional (3-D) growth pattern of endometrial cells using a 3-D in vitro culture system.METHODS:
Isolated endometrial epithelial cells, stromal cells and RL95-2 cells were seeded into culture chambers coated with the extracellular matrix Matrigel and observed using light microscopy. Fluorescence staining and immunohistochemistry were used to assess the morphology.RESULTS:
Depending on the culture conditions, epithelial cells and RL95-2 cells formed multicellular structures on Matrigel; stromal cells remained individually distinguishable or grew together to form 3-D lattice-like structures.CONCLUSIONS:
Matrigel provided a good microenvironment for culturing endometrial cells. The cells cultured in the Matrigel-coated chambers closely resembled those seen in vivo.
Buscar en Google
Banco de datos:
MEDLINE
Asunto principal:
Técnicas de Cultivo de Célula
/
Endometrio
Límite:
Female
/
Humans
Idioma:
En
Año:
2012
Tipo del documento:
Article