Targeting both rs12979860 and rs8099917 polymorphisms with a single-tube high-resolution melting assay for IL28b genotyping.
J Clin Microbiol
; 50(10): 3353-5, 2012 Oct.
Article
en En
| MEDLINE
| ID: mdl-22814458
ABSTRACT
A rapid, duplex, high-resolution melting interleukin-28B gene (IL28B) genotyping assay, targeting both rs12979860 and rs8099917 polymorphisms, was developed and validated using 30 DNA samples from healthy volunteers. A linkage study on 300 healthy Singaporeans showed variable haplotypes. When the assay was applied to plasma DNA from 50 hepatitis C virus genotype-1 (HCV-1)-infected patients, five compound heterozygous types were detected.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Interleucinas
/
Hepatitis C
/
Técnicas de Laboratorio Clínico
/
Polimorfismo de Nucleótido Simple
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Técnicas de Diagnóstico Molecular
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
En
Año:
2012
Tipo del documento:
Article