Long-term culture-initiating cell assay for mouse cells.
Methods Mol Biol
; 946: 257-66, 2013.
Article
en En
| MEDLINE
| ID: mdl-23179837
ABSTRACT
The long-term culture-initiating cell (LTC-IC) assay is a well-established in vitro assay used to enumerate primitive mouse hematopoietic stem cells (HSCs) and relies on the two cardinal functions of HSCs ability to self-renew and differentiation capacity. LTC-ICs present in minimally processed and purified cell suspensions and cocultured on a supportive feeder layer are detected by their sustained ability to produce hematopoietic progenitors (colony forming cells) after ≥ 4 weeks in culture. Refinements including the use of a defined stromal cell line, and extending the in vitro culture to 6 weeks allow detection of LTC-IC at similar frequencies to transplantable HSCs quantified using in vivo assays.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Células Madre Hematopoyéticas
/
Ensayo de Unidades Formadoras de Colonias
Límite:
Animals
Idioma:
En
Año:
2013
Tipo del documento:
Article