Reaction products of 1-naphthol with reactive oxygen species prevent NADPH oxidase activation in activated human neutrophils, but leave phagocytosis intact.
Free Radic Biol Med
; 8(3): 241-9, 1990.
Article
en En
| MEDLINE
| ID: mdl-2341054
ABSTRACT
Activation of human neutrophils with opsonized particles in the presence of a nontoxic dose of 1-naphthol resulted in inhibition of superoxide anion production but not of the phagocytotic activity of the cells. In this study we have investigated the mechanism of action of 1-naphthol. The inhibition is not at the level of cellular activation since the FMLP-induced rise of intracellular free calcium was unaffected. Our results show that the (metabolic) activation of 1-naphthol to 1,4-naphthoquinone by reaction with H2O2 from the oxidative burst is a necessary event for the inhibition to occur. The study provides evidence that by its reactivity with essential thiol groups 1,4-naphthoquinone (1,4-NQ) prevents the assembly of a functional NADPH-oxidase in the neutrophil membrane.
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Banco de datos:
MEDLINE
Asunto principal:
Fagocitosis
/
NADH NADPH Oxidorreductasas
/
Naftoles
/
Neutrófilos
Límite:
Humans
Idioma:
En
Año:
1990
Tipo del documento:
Article