Metformin induces up-regulation of blood-brain barrier functions by activating AMP-activated protein kinase in rat brain microvascular endothelial cells.
Biochem Biophys Res Commun
; 433(4): 586-90, 2013 Apr 19.
Article
en En
| MEDLINE
| ID: mdl-23523792
ABSTRACT
Blood-brain barrier (BBB) disruption occurs frequently in CNS diseases and injuries. Few drugs have been developed as therapeutic candidates for facilitating BBB functions. Here, we examined whether metformin up-regulates BBB functions using rat brain microvascular endothelial cells (RBECs). Metformin, concentration- and time-dependently increased transendothelial electrical resistance of RBEC monolayers, and decreased RBEC permeability to sodium fluorescein and Evans blue albumin. These effects of metformin were blocked by compound C, an inhibitor of AMP-activated protein kinase (AMPK). AMPK stimulation with an AMPK activator, AICAR, enhanced BBB functions. These findings indicate that metformin induces up-regulation of BBB functions via AMPK activation.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Encéfalo
/
Barrera Hematoencefálica
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Regulación hacia Arriba
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Adenilato Quinasa
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Metformina
Límite:
Animals
Idioma:
En
Año:
2013
Tipo del documento:
Article