Entire sequence of the colonization factor coli surface antigen 6-encoding plasmid pCss165 from an enterotoxigenic Escherichia coli clinical isolate.
Plasmid
; 70(3): 343-52, 2013 Nov.
Article
en En
| MEDLINE
| ID: mdl-23933356
ABSTRACT
Coli surface antigen 6 (CS6) is one of the most prevalent colonization factors among enterotoxigenic Escherichia coli (ETEC) isolated in developing countries. Although it is known that CS6 is encoded by a plasmid, there are no reports on the sequence analysis of the CS6-encoding plasmid or genes exhibiting similar behavior to CS6. Here, we report the isolation of the CS6-encoding plasmid, pCss165Kan, from 4266 ΔcssBkanamycin (Km) and its complete nucleotide sequence. This plasmid consisted of 165,311bp and 222 predicted coding sequences. Remarkably, there were many insertion sequence (IS) elements, which comprised 24.4% of the entire sequence. Virulence-associated genes such as heat-stable enterotoxin, homologues of ATP-binding cassette transporter in enteroaggregative E. coli (EAEC), and ETEC autotransporter A were also present, although the ETEC autotransporter A gene was disrupted by the integration of IS629. We found that 2 transcriptional regulators belonging to the AraC family were not involved in CS6 expression. Interestingly, pCss165 had conjugative transfer genes, as well as 3 toxin-antitoxin systems that potentially exclude other plasmid-free host bacteria. These genes might be involved in the prevalence of CS6 among ETEC isolates.
Palabras clave
BLAST; CDSs; CFA; CFs; CS; CS6; Colonization factor; DIG; Enterotoxigenic Escherichia coli; IS; Km and Kan; PBS; PHD; ST; Virulence plasmid; basic local alignment search tool; coding sequences; coli surface antigen; colonization factor antigen; colonization factors; digoxigenin; heat-stable enterotoxin; insertion sequence; kanamycin; phosphate buffered saline; prevent host death
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Plásmidos
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Regulación Bacteriana de la Expresión Génica
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Proteínas de Escherichia coli
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Proteínas Fimbrias
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Factores de Virulencia
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Escherichia coli Enterotoxigénica
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Antígenos Bacterianos
Tipo de estudio:
Prognostic_studies
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Risk_factors_studies
Límite:
Humans
Idioma:
En
Año:
2013
Tipo del documento:
Article