Detection of S-nitrosothiols.
Biochim Biophys Acta
; 1840(2): 892-900, 2014 Feb.
Article
en En
| MEDLINE
| ID: mdl-23988402
ABSTRACT
BACKGROUND:
S-nitrosothiols have been recognized as biologically-relevant products of nitric oxide that are involved in many of the diverse activities of this free radical. SCOPE OF REVIEW This review serves to discuss current methods for the detection and analysis of protein S-nitrosothiols. The major methods of S-nitrosothiol detection include chemiluminescence-based methods and switch-based methods, each of which comes in various flavors with advantages and caveats. MAJORCONCLUSIONS:
The detection of S-nitrosothiols is challenging and prone to many artifacts. Accurate measurements require an understanding of the underlying chemistry of the methods involved and the use of appropriate controls. GENERALSIGNIFICANCE:
Nothing is more important to a field of research than robust methodology that is generally trusted. The field of S-nitrosation has developed such methods but, as S-nitrosothiols are easy to introduce as artifacts, it is vital that current users learn from the lessons of the past. This article is part of a Special Issue entitled Current methods to study reactive oxygen species - pros and cons and biophysics of membrane proteins. Guest Editor Christine Winterbourn.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
S-Nitrosotioles
/
Mediciones Luminiscentes
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Año:
2014
Tipo del documento:
Article