Hematopoietic stem cell expansion caused by a synthetic fragment of leptin.
Peptides
; 50: 24-7, 2013 Dec.
Article
en En
| MEDLINE
| ID: mdl-24090593
ABSTRACT
Leptin is a cytokine that regulates food intake, energy expenditure and hematopoiesis. Based on the tridimensional structure of the human leptin molecule, six fragments have been synthesized, (Ac-Lep23-47-NH2, [LEP1]; Ac-Lep48-71-NH2, [LEP2]; Ac-Lep72-88-NH2, [LEP3]; Ac-Lep92-115-NH2, [LEP4], Ac-[Ser(117)]-Lep116-140-NH2, [LEP5] and Ac-Lep141-164-NH2, [LEP6]), and their effects on hematopoiesis were evaluated. The mice were treated with 1mg/kg LEP5 for 3 days. The mature and primitive hematopoietic populations were quantified. We observed that the mature populations from the bone marrow and spleen were not affected by LEP5. However, the peptide caused at least a two-fold increase in the number of hematopoietic stem cells, the most primitive population of the bone marrow. Additionally, the number of granulocyte/macrophage colony-forming units produced by bone marrow cells in methylcellulose also increased by 40% after treatment with LEP5, and the leptin receptor was activated. These results show that the leptin fragment LEP5 is a positive modulator of the in vivo expansion of hematopoietic stem cells.
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1
Banco de datos:
MEDLINE
Asunto principal:
Fragmentos de Péptidos
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Bazo
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Células de la Médula Ósea
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Células Madre Hematopoyéticas
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Leptina
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Hematopoyesis
Límite:
Animals
Idioma:
En
Año:
2013
Tipo del documento:
Article