Association of homocysteine with global DNA methylation in vegetarian Indian pregnant women and neonatal birth anthropometrics.
J Matern Fetal Neonatal Med
; 27(17): 1749-53, 2014 Nov.
Article
en En
| MEDLINE
| ID: mdl-24397861
ABSTRACT
OBJECTIVE:
The present study was designed to evaluate if plasma maternal folate, vitamin B-12 and homocysteine levels had an effect on maternal global DNA methylation and neonatal anthropometrics in Indian pregnant women.METHODS:
A total of 49 participants having completed ≥36 weeks of pregnancy were enrolled in the study. Estimation of folate was by Ion capture assay, vitamin B-12 by microparticle enzyme immunoassay, total homocysteine by fluorescence polarization immunoassay and global DNA methylation using Cayman's DNA methylation enzyme immunoassay (EIA) kit.RESULTS:
Folate and vitamin B-12 were inversely correlated to homocysteine in pregnant women consuming vegetarian and non-vegetarian diet. No difference in global DNA methylation was found between the vegetarian and non-vegetarian pregnant women. Folate and vitamin B-12 did not show association with global DNA methylation, however plasma total homocysteine of the vegetarian group showed significant correlation to global DNA methylation (r(2 )= 0.49, p = 0.011). Plasma total homocysteine was inversely related to tricep skinfold (r(2 )= -0.484, p = 0.01) and chest circumference (r(2 )= -0.104, p = 0.04) of neonates in vegetarian group.CONCLUSION:
Moderate vitamin B-12 deficiency in vegetarian pregnant women might be the cause of hyperhomocystinemia, hypermethylation when compared to vitamin B-12 sufficient non-vegetarian group.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Dieta Vegetariana
/
Peso al Nacer
/
Estado Nutricional
/
Metilación de ADN
/
Fenómenos Fisiologicos Nutricionales Maternos
/
Homocisteína
Tipo de estudio:
Risk_factors_studies
Límite:
Adult
/
Female
/
Humans
/
Newborn
/
Pregnancy
País/Región como asunto:
Asia
Idioma:
En
Año:
2014
Tipo del documento:
Article