Prevalence, identification by a DNA microarray-based assay of human and food isolates Listeria spp. from Tunisia.
Pathol Biol (Paris)
; 62(1): 24-9, 2014 Feb.
Article
en En
| MEDLINE
| ID: mdl-24461393
ABSTRACT
OBJECTIVES:
We aimed at evaluating the prevalence of Listeria species isolated from food samples and characterizing food and human cases isolates. MATERIAL ANDMETHODS:
Between 2005 and 2007, one hundred food samples collected in the markets of Tunis were analysed in our study. Five strains of Listeria monocytogenes responsible for human listeriosis isolated in hospital of Tunis were included. Multiplex PCR serogrouping and pulsed field gel electrophoresis (PFGE) applying the enzyme AscI and ApaI were used for the characterization of isolates of L. monocytogenes. We have developed a rapid microarray-based assay to a reliable discrimination of species within the Listeria genus.RESULTS:
The prevalence of Listeria spp. in food samples was estimated at 14% by using classical biochemical identification. Two samples were assigned to L. monocytogenes and 12 to L. innocua. DNA microarray allowed unambiguous identification of Listeria species. Our results obtained by microarray-based assay were in accordance with the biochemical identification. The two food L. monocytogenes isolates were assigned to the PCR serogroup IIa (serovar 1/2a). Whereas human L. monocytogenes isolates were of PCR serogroup IVb, (serovars 4b). These isolates present a high similarity in PFGE. Food L. monocytogenes isolates were classified into two different pulsotypes. These pulsotypes were different from that of the five strains responsible for the human cases.CONCLUSION:
We confirmed the presence of Listeria spp. in variety of food samples in Tunis. Increased food and clinical surveillance must be taken into consideration in Tunisia to identify putative infections sources.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Técnicas de Tipificación Bacteriana
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Análisis de Secuencia por Matrices de Oligonucleótidos
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Microbiología de Alimentos
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Listeria
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Listeriosis
Tipo de estudio:
Diagnostic_studies
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Prevalence_studies
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Prognostic_studies
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Risk_factors_studies
Límite:
Aged80
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Animals
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Female
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Humans
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Infant
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Male
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Newborn
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Pregnancy
País/Región como asunto:
Africa
Idioma:
En
Año:
2014
Tipo del documento:
Article