Interest of human papillomavirus DNA quantification and genotyping in paired cervical and urine samples to detect cervical lesions.
Arch Gynecol Obstet
; 290(2): 299-308, 2014 Aug.
Article
en En
| MEDLINE
| ID: mdl-24622934
ABSTRACT
BACKGROUND:
Cervical cancer is caused by persistent infection with high-risk human papillomavirus (HR-HPV). Conventional human papillomavirus (HPV) testing requires cervical sampling. However, vaginal and urine self-sampling methods are more acceptable for patients and result in increased participation when they are available in screening programs. In this context, we have developed a non-invasive screening method via the detection of HPV DNA in urine samples.PURPOSE:
To compare HPV viral loads and genotypes in paired cervical and urine samples, and to assess correlation between virological and cytological results in women seeking gynecological consultation.METHODS:
Paired urine and cervical specimens were collected and analyzed from 230 of 245 women participating in the previously described prospective PapU study. HPV DNA detection and quantification were performed using a real-time PCR method with short fragment PCR primers. Genotyping was carried out using the INNO-LiPA HPV genotyping assay.RESULTS:
The prevalence of HPV in the 230 paired urine and cervical smear samples was 42 and 49 %, respectively. Overall agreement for HPV positivity and negativity between the paired samples was 90 % (κ = 0.80). High HPV viral load in both cervical and urine samples was associated with cytological abnormalities. HPV-positive women were mostly infected with HR-HPV types. The agreement between high- and low-risk HPV (LR-HPV) detection in both samples was 97 % (κ = 0.95 for HR-HPV and κ = 0.97 for LR-HPV).CONCLUSIONS:
High concordance rates for HPV-DNA quantification and high/low-risk HPV genotyping in paired urine/cervical samples suggest that urinary HPV DNA testing could be useful for cervical lesion screening.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Papillomaviridae
/
ADN Viral
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Neoplasias del Cuello Uterino
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Cuello del Útero
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Infecciones por Papillomavirus
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Pruebas de ADN del Papillomavirus Humano
Tipo de estudio:
Clinical_trials
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Diagnostic_studies
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Observational_studies
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Prevalence_studies
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Risk_factors_studies
Límite:
Adolescent
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Adult
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Aged
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Female
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Humans
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Middle aged
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Pregnancy
País/Región como asunto:
Europa
Idioma:
En
Año:
2014
Tipo del documento:
Article