The identification of wadB, a new glycosyltransferase gene, confirms the branched structure and the role in virulence of the lipopolysaccharide core of Brucella abortus.
Microb Pathog
; 73: 53-9, 2014 Aug.
Article
en En
| MEDLINE
| ID: mdl-24927935
ABSTRACT
Brucellosis is a worldwide extended zoonosis caused by Brucella spp. These gram-negative bacteria are not readily detected by innate immunity, a virulence-related property largely linked to their surface lipopolysaccharide (LPS). The role of the LPS lipid A and O-polysaccharide in virulence is well known. Moreover, mutation of the glycosyltransferase gene wadC of Brucella abortus, although not affecting O-polysaccharide assembly onto the lipid-A core section causes a core oligosaccharide defect that increases recognition by innate immunity. Here, we report on a second gene (wadB) encoding a LPS core glycosyltransferase not involved in the assembly of the O-polysaccharide-linked core section. As compared to wild-type B. abortus, a wadB mutant was sensitive to bactericidal peptides and non-immune serum, and was attenuated in mice and dendritic cells. These observations show that as WadC, WadB is also involved in the assembly of a branch of Brucella LPS core and support the concept that this LPS section is a virulence-related structure.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Brucella abortus
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Lipopolisacáridos
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Glicosiltransferasas
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Año:
2014
Tipo del documento:
Article