Detection of yellow fever viral RNA by nucleic acid hybridization and viral antigen by immunocytochemistry in fixed human liver.
Am J Trop Med Hyg
; 40(6): 663-8, 1989 Jun.
Article
en En
| MEDLINE
| ID: mdl-2500857
ABSTRACT
Histopathologic examination of liver from patients with yellow fever is often not diagnostic. We therefore compared 2 virus-specific assays applicable to fixed liver, in situ nucleic acid hybridization and an immunocytochemical [alkaline phosphatase-antialkaline phosphatase (APAAP)] technique. Yellow fever structural gene sequences were detected by use of 35S-labeled negative-sense RNA probe (but not by immunocytochemistry) in 11 of 17 livers from children with fatal illness during the 1965 epidemic in Senegal. These fixed liver samples had been stored at ambient temperatures for 23 years. Both techniques were diagnostic on tissues collected 15-37 months before testing. Immunocytochemistry is a practical procedure for rapid specific diagnosis of liver stored for months, whereas RNA-RNA hybridization is a sensitive technique which can be applied to material stored for years.
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Banco de datos:
MEDLINE
Asunto principal:
Virus de la Fiebre Amarilla
/
ARN Viral
/
Hígado
Tipo de estudio:
Diagnostic_studies
Límite:
Adolescent
/
Animals
/
Child
/
Child, preschool
/
Female
/
Humans
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Infant
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Male
/
Middle aged
Idioma:
En
Año:
1989
Tipo del documento:
Article