CD38 expression in early B-cell precursors contributes to extracellular signal-regulated kinase-mediated apoptosis.
Immunology
; 144(2): 271-81, 2015 Feb.
Article
en En
| MEDLINE
| ID: mdl-25155483
ABSTRACT
CD38 is a 45,000 molecular weight transmembrane protein that is expressed in immature and mature lymphocytes. However, the expression and function of CD38 during B-cell differentiation in mice is poorly understood. Here, we report that CD38 is expressed from the earliest stages of B-cell development. Pre-pro-B, pro-B, pre-B and immature B cells from murine bone marrow all stained positive for CD38. Interestingly, CD38 expression increases with B-cell maturation. To assess the role of CD38 during B-cell maturation, CD38-deficient mice were analysed. CD38(-/-) mice showed a significant increase in both the frequency of B-lineage cells and the absolute numbers of pre-pro-B cells in bone marrow; however, no other differences were observed at later stages. CD38 cross-linking in Ba/F3 cells promoted apoptosis and marked extracellular signal-regulated kinase (ERK) phosphorylation, and these effects were reduced by treatment with the mitogen-activated protein kinase/ERK kinase inhibitor PD98059, and similar effects were observed in B-cell precursors from bone marrow. These data demonstrate that B-cell precursors in mouse bone marrow express functional CD38 and implicate the early ligation of CD38 in the ERK-associated regulation of the B-lineage differentiation pathway.
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1
Banco de datos:
MEDLINE
Asunto principal:
Células de la Médula Ósea
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Glicoproteínas de Membrana
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Quinasas MAP Reguladas por Señal Extracelular
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ADP-Ribosil Ciclasa 1
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Células Precursoras de Linfocitos B
Límite:
Animals
Idioma:
En
Año:
2015
Tipo del documento:
Article