Archaerhodopsin variants with enhanced voltage-sensitive fluorescence in mammalian and Caenorhabditis elegans neurons.
Nat Commun
; 5: 4894, 2014 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-25222271
ABSTRACT
Probing the neural circuit dynamics underlying behaviour would benefit greatly from improved genetically encoded voltage indicators. The proton pump Archaerhodopsin-3 (Arch), an optogenetic tool commonly used for neuronal inhibition, has been shown to emit voltage-sensitive fluorescence. Here we report two Arch variants with enhanced radiance (Archers) that in response to 655 nm light have 3-5 times increased fluorescence and 55-99 times reduced photocurrents compared with Arch WT. The most fluorescent variant, Archer1, has 25-40% fluorescence change in response to action potentials while using 9 times lower light intensity compared with other Arch-based voltage sensors. Archer1 is capable of wavelength-specific functionality as a voltage sensor under red light and as an inhibitory actuator under green light. As a proof-of-concept for the application of Arch-based sensors in vivo, we show fluorescence voltage sensing in behaving Caenorhabditis elegans. Archer1's characteristics contribute to the goal of all-optical detection and modulation of activity in neuronal networks in vivo.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Potenciales de Acción
/
Proteínas del Helminto
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Proteínas Arqueales
/
Proteínas del Tejido Nervioso
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Neuronas
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Año:
2014
Tipo del documento:
Article