Modified P elements that mimic the P cytotype in Drosophila melanogaster.
Genetics
; 123(4): 815-24, 1989 Dec.
Article
en En
| MEDLINE
| ID: mdl-2558959
ABSTRACT
Activity of the P family of transposable elements in Drosophila melanogaster is regulated primarily by a cellular condition known as P cytotype. It has been hypothesized that P cytotype depends on a P element-encoded repressor of transposition and excision. We provide evidence in support of this idea by showing that two modified P elements, each with lesions affecting the fourth transposase exon, mimic most of the P cytotype effects. These elements were identified by means of two sensitive assays capable of detecting repression by a single P element. One assay makes use of cytotype-dependent gene expression of certain P element insertion mutations at the singed bristle locus. The other measures suppression of transposase activity from the unusually stable genomic P element, delta 2-3(99B), that normally produces transposase in both germinal and somatic tissues. The P cytotype-like effects include suppression of snw germline hypermutability, snw somatic mosaicism, pupal lethality, and gonadal dysgenic sterility. Unlike P cytotype, however, there was no reciprocal cross effect in the inheritance of repression.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Elementos Transponibles de ADN
/
Drosophila melanogaster
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Año:
1989
Tipo del documento:
Article