A lab-on-a-chip system with integrated sample preparation and loop-mediated isothermal amplification for rapid and quantitative detection of Salmonella spp. in food samples.
Lab Chip
; 15(8): 1898-904, 2015 Apr 21.
Article
en En
| MEDLINE
| ID: mdl-25715949
ABSTRACT
Foodborne disease is a major public health threat worldwide. Salmonellosis, an infectious disease caused by Salmonella spp., is one of the most common foodborne diseases. Isolation and identification of Salmonella by conventional bacterial culture or molecular-based methods are time consuming and usually take a few hours to days to complete. In response to the demand for rapid on line or on site detection of pathogens, in this study, we describe for the first time an eight-chamber lab-on-a-chip (LOC) system with integrated magnetic bead-based sample preparation and loop-mediated isothermal amplification (LAMP) for rapid and quantitative detection of Salmonella spp. in food samples. The whole diagnostic procedures including DNA isolation, isothermal amplification, and real-time detection were accomplished in a single chamber. Up to eight samples could be handled simultaneously and the system was capable to detect Salmonella at concentration of 50 cells per test within 40 min. The simple design, together with high level of integration, isothermal amplification, and quantitative analysis of multiple samples in short time, will greatly enhance the practical applicability of the LOC system for rapid on-site screening of Salmonella for applications in food safety control, environmental surveillance, and clinical diagnostics.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Integración de Sistemas
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Salmonella enterica
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Técnicas de Amplificación de Ácido Nucleico
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Métodos Analíticos de la Preparación de la Muestra
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Dispositivos Laboratorio en un Chip
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Microbiología de Alimentos
Tipo de estudio:
Diagnostic_studies
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Prognostic_studies
Idioma:
En
Año:
2015
Tipo del documento:
Article