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Detection of a New Luteovirus in Imported Nectarine Trees: A Case Study to Propose Adoption of Metagenomics in Post-Entry Quarantine.
Bag, Sudeep; Al Rwahnih, Maher; Li, Ashley; Gonzalez, Asaul; Rowhani, Adib; Uyemoto, Jerry K; Sudarshana, Mysore R.
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  • Bag S; First, second, and fifth authors: Department of Plant Pathology, University of California, One Shields Avenue, Davis 95616; and third, fourth, sixth, and seventh authors: U.S. Department of Agriculture, Agricultural Research Service, Department of Plant Pathology, University of California, One Shiel
  • Al Rwahnih M; First, second, and fifth authors: Department of Plant Pathology, University of California, One Shields Avenue, Davis 95616; and third, fourth, sixth, and seventh authors: U.S. Department of Agriculture, Agricultural Research Service, Department of Plant Pathology, University of California, One Shiel
  • Li A; First, second, and fifth authors: Department of Plant Pathology, University of California, One Shields Avenue, Davis 95616; and third, fourth, sixth, and seventh authors: U.S. Department of Agriculture, Agricultural Research Service, Department of Plant Pathology, University of California, One Shiel
  • Gonzalez A; First, second, and fifth authors: Department of Plant Pathology, University of California, One Shields Avenue, Davis 95616; and third, fourth, sixth, and seventh authors: U.S. Department of Agriculture, Agricultural Research Service, Department of Plant Pathology, University of California, One Shiel
  • Rowhani A; First, second, and fifth authors: Department of Plant Pathology, University of California, One Shields Avenue, Davis 95616; and third, fourth, sixth, and seventh authors: U.S. Department of Agriculture, Agricultural Research Service, Department of Plant Pathology, University of California, One Shiel
  • Uyemoto JK; First, second, and fifth authors: Department of Plant Pathology, University of California, One Shields Avenue, Davis 95616; and third, fourth, sixth, and seventh authors: U.S. Department of Agriculture, Agricultural Research Service, Department of Plant Pathology, University of California, One Shiel
  • Sudarshana MR; First, second, and fifth authors: Department of Plant Pathology, University of California, One Shields Avenue, Davis 95616; and third, fourth, sixth, and seventh authors: U.S. Department of Agriculture, Agricultural Research Service, Department of Plant Pathology, University of California, One Shiel
Phytopathology ; 105(6): 840-6, 2015 Jun.
Article en En | MEDLINE | ID: mdl-25775105
ABSTRACT
In spring 2013, 5-year-old nectarine (Prunus persica) trees, grafted on peach rootstock Nemaguard, were found stunted in a propagation block in California. These trees had been propagated from budwood of three nectarine cultivars imported from France and cleared through the post-entry quarantine procedure. Examination of the canopy failed to reveal any obvious symptoms. However, examination of the trunks, after stripping the bark, revealed extensive pitting on the woody cylinder. To investigate the etiological agent, double-stranded RNA was extracted from bark scrapings from the scion and rootstock portions, and a cDNA library was prepared and sequenced using the Illumina platform. BLAST analysis of the contigs generated by the de novo assembly of sequence reads indicated the presence of a novel luteovirus. Complete sequence of the viral genome was determined by sequencing of three overlapping cDNA clones generated by reverse transcription-polymerase chain reaction (RT-PCR) and by rapid amplification of the 5'- and 3'-termini. The virus genome was comprised of 4,991 nucleotides with a gene organization similar to members of the genus Luteovirus (family Luteoviridae). The presence of the virus, tentatively named Nectarine stem pitting-associated virus, was confirmed in symptomatic trees by RT-PCR. Discovery of a new virus in nectarine trees after post-entry quarantine indicates the importance of including (i) metagenomic analysis by next-generation sequencing approach as an essential tool to assess the plant health status, and (ii) examination of the woody cylinders as part of the indexing process.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Genoma Viral / Luteovirus / Prunus / Metagenómica Tipo de estudio: Diagnostic_studies País/Región como asunto: America do norte / Europa Idioma: En Año: 2015 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Genoma Viral / Luteovirus / Prunus / Metagenómica Tipo de estudio: Diagnostic_studies País/Región como asunto: America do norte / Europa Idioma: En Año: 2015 Tipo del documento: Article