Purification and characterization of four antibacterial peptides from protamex hydrolysate of Atlantic mackerel (Scomber scombrus) by-products.
Biochem Biophys Res Commun
; 462(3): 195-200, 2015 Jul 03.
Article
en En
| MEDLINE
| ID: mdl-25934151
ABSTRACT
Proteins from fish by-product sources are valuable source of bioactive peptides and show promise as functional foods ingredients. The objective of the present study was to isolate and characterize antibacterial peptides from protamex hydrolysates of Atlantic mackerel (Scomber scombrus) by-products. Four sequences SIFIQRFTT (P4), RKSGDPLGR (P8.1), AKPGDGAGSGPR (P8.2) and GLPGPLGPAGPK (P11) were identified in peptide fractions separated using RP-HPLC. At 200 µg mL(-1), while peptides P8.1, P8.2 and P11 exhibited partial inhibition, P4 totally inhibited tested Gram-positive (Listeria innocua) and Gram-negative (Escherichia coli) bacterial strains. These results suggest that the protein hydrolysate derived from mackerel by-products could be used as an antimicrobial ingredient in both functional food and nutraceutical applications.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Perciformes
/
Péptidos Catiónicos Antimicrobianos
/
Proteínas de Peces
/
Antibacterianos
Límite:
Animals
Idioma:
En
Año:
2015
Tipo del documento:
Article