High Yield Non-detergent Isolation of Photosystem I-Light-harvesting Chlorophyll II Membranes from Spinach Thylakoids: IMPLICATIONS FOR THE ORGANIZATION OF THE PS I ANTENNAE IN HIGHER PLANTS.
J Biol Chem
; 290(30): 18429-37, 2015 Jul 24.
Article
en En
| MEDLINE
| ID: mdl-26055710
ABSTRACT
Styrene-maleic acid copolymer was used to effect a non-detergent partial solubilization of thylakoids from spinach. A high density membrane fraction, which was not solubilized by the copolymer, was isolated and was highly enriched in the Photosystem (PS) I-light-harvesting chlorophyll (LHC) II supercomplex and depleted of PS II, the cytochrome b6/f complex, and ATP synthase. The LHC II associated with the supercomplex appeared to be energetically coupled to PS I based on 77 K fluorescence, P700 photooxidation, and PS I electron transport light saturation experiments. The chlorophyll (Chl) a/b ratio of the PS I-LHC II membranes was 3.2 ± 0.9, indicating that on average, three LHC II trimers may associate with each PS I. The implication of these findings within the context of higher plant PS I antenna organization is discussed.
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Banco de datos:
MEDLINE
Asunto principal:
Fotosíntesis
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Cloroplastos
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Complejos de Proteína Captadores de Luz
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Complejo de Citocromo b6f
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Complejo de Proteína del Fotosistema I
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Complejo de Proteína del Fotosistema II
Idioma:
En
Año:
2015
Tipo del documento:
Article