Generation and Characterization of Patient-Specific iPSC Model for Cardiovascular Disease.
Methods Mol Biol
; 1353: 191-213, 2016.
Article
en En
| MEDLINE
| ID: mdl-26126449
ABSTRACT
Advances in differentiation of cardiomyocytes from human induced pluripotent stem cell (hiPSC) were emerged as a tool for modeling of cardiovascular disease that recapitulates the phenotype for the purpose of drug screening, biomarker discovery, and testing of single-nucleotide polymorphism (SNP) as a modifier for disease stratification. Here, we describe the (1) retroviral reprogramming strategies in the generation of human iPSC, (2) methodology in characterization of iPSC in order to identify the stem cell clones with the best quality, and (3) protocol of cardiac differentiation by modulation of Wnt signaling and ß-catenin pathway.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Técnicas de Cultivo de Célula
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Miocitos Cardíacos
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Reprogramación Celular
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Células Madre Pluripotentes Inducidas
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Cuerpos Embrioides
Tipo de estudio:
Prognostic_studies
Idioma:
En
Año:
2016
Tipo del documento:
Article