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Influence of selective digestion of elastin and collagen on mechanical properties of human aortas.
Kobielarz, Magdalena; Chwilkowska, Agnieszka; Turek, Artur; Maksymowicz, Krzysztof; Marciniak, Monika.
Afiliación
  • Kobielarz M; Department of Biomedical Engineering, Mechatronics and Theory of Mechanisms, Wroclaw University of Technology, Wroclaw, Poland.
  • Chwilkowska A; Regional Specialist Hospital in Wroclaw, Research and Development Centre, Wroclaw, Poland.
  • Turek A; Department of Medical Biochemistry, Medical University, Wroclaw, Poland.
  • Maksymowicz K; School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Poland, Chair and Department of Biopharmacy, Sosnowiec, Poland.
  • Marciniak M; Centre of Polymer and Carbon Materials, Polish Academy of Sciences, Zabrze, Poland.
Acta Bioeng Biomech ; 17(2): 55-62, 2015.
Article en En | MEDLINE | ID: mdl-26415712
ABSTRACT

PURPOSE:

There are two families of fibres taking part in the process of mechanical loads transfer, i.e. elastin and collagen fibres. Their number, spatial arrangement and specific properties determine the capacity of a blood vessels to resist mechanical loads resulting from the impact of blood on vessel walls. The purpose of the present paper is to define the load-bearing capacities of elastin and collagen scaffolds equivalent to natural fibre arrangements of human aorta and produced by selective digestion.

METHODS:

Samples of thoracic human aortas were digested by using phosphate buffer of trypsin at pH 8.0 for 22 hours in order to degrade elastin and by autoclaving followed by incubation in 90% formic acid for 22 hours. The efficacy of digestion was assessed immunohistochemically. Mechanical properties of pre-stretched native and digested samples were determined by uniaxial tensile test.

RESULTS:

Samples subjected to autoclaving have been successfully deprived of both types of collagen and elastin has been intact. Treatment with trypsin caused a removal of elastin and the presence of type I and IV collagen was demonstrated. Digestion of aortic samples either by formic acid or trypsin has resulted significantly decreasing mechanical properties in comparison with native samples.

CONCLUSIONS:

Collagen and elastin scaffold-like stuctures have been effectively produced by selective digestion of thoracic human aorta and their contribution to the load-bearing process was evaluated. Isolated collagen network are more durable and stiffer and less deformable than elastin network, hence are responsible for load-bearing process at higher strain since the range of working of elastin is at lower strain values.
Asunto(s)
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Banco de datos: MEDLINE Asunto principal: Aorta Torácica / Colágeno / Elastina / Soporte de Peso / Mecanotransducción Celular / Modelos Cardiovasculares Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Año: 2015 Tipo del documento: Article
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Banco de datos: MEDLINE Asunto principal: Aorta Torácica / Colágeno / Elastina / Soporte de Peso / Mecanotransducción Celular / Modelos Cardiovasculares Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Año: 2015 Tipo del documento: Article