Systemic control of protein synthesis through sequestration of translation and ribosome biogenesis factors during severe heat stress.
FEBS Lett
; 589(23): 3654-64, 2015 Nov 30.
Article
en En
| MEDLINE
| ID: mdl-26484595
ABSTRACT
Environmental stress causes the sequestration of proteins into insoluble deposits including cytoplasmic stress granules (SGs), containing mRNA and a variety of translation factors. Here we systematically identified proteins sequestered in Saccharomyces cerevisiae at 46 °C by a SG co-localization screen and proteomic analysis of insoluble protein fractions. We identified novel SG components including essential aminoacyl-tRNA synthetases. Moreover, we discovered nucleus-associated deposits containing ribosome biogenesis factors. Our study suggests downregulation of cytosolic protein synthesis and nuclear ribosome production at multiple levels through heat shock induced protein sequestrations.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Ribosomas
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Biogénesis de Organelos
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Respuesta al Choque Térmico
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Proteínas de Saccharomyces cerevisiae
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Proteómica
Tipo de estudio:
Prognostic_studies
Idioma:
En
Año:
2015
Tipo del documento:
Article