Identification and characterization of the zosA gene involved in copper uptake in Bacillus subtilis 168.
Biosci Biotechnol Biochem
; 80(3): 600-9, 2016.
Article
en En
| MEDLINE
| ID: mdl-26566138
ABSTRACT
DL-Penicillamine, a copper-specific metal chelator, remarkably suppressed the growth of Bacillus subtilis 168 when added to a synthetic medium under Cu(2+) limitation. DNA microarray and screening of 2,602 knockout mutants showed that the zosA gene was de-repressed in the presence of 0.1% dl-penicillamine, and that the zosA mutant was sensitive to dl-penicillamine medium. The zosA mutant delayed the growth under Cu-limitation even without the chelator, and the sensitivity to dl-penicillamine was reversed by induction using 0.3 mM IPTG and the Pspac promoter inserted directly upstream of the zosA gene. Furthermore, the zosA mutant showed elevated tolerance of excessive Cu(2+) but not of excessive Zn(2+) added to LB and synthetic media. Homology modeling of the ZosA protein suggested that the protein can fold itself into essential domains for constituting a metal transporting ATPase. Our study suggests that zosA is a candidate gene involved in copper uptake.
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Banco de datos:
MEDLINE
Asunto principal:
Bacillus subtilis
/
Cobre
/
Genes Bacterianos
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Año:
2016
Tipo del documento:
Article