[Efficient genome editing in human pluripotent stem cells through CRISPR/Cas9].
Yi Chuan
; 37(11): 1167-73, 2015 11.
Article
en Zh
| MEDLINE
| ID: mdl-26582531
ABSTRACT
The RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has offered a new platform for genome editing with high efficiency. Here, we report the use of CRISPR/Cas9 technology to target a specific genomic region in human pluripotent stem cells. We show that CRISPR/Cas9 can be used to disrupt a gene by introducing frameshift mutations to gene coding region; to knock in specific sequences (e.g. FLAG tag DNA sequence) to targeted genomic locus via homology directed repair; to induce large genomic deletion through dual-guide multiplex. Our results demonstrate the versatile application of CRISPR/Cas9 in stem cell genome editing, which can be widely utilized for functional studies of genes or genome loci in human pluripotent stem cells.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Genoma Humano
/
Edición de ARN
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Células Madre Pluripotentes
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Sistemas CRISPR-Cas
Límite:
Humans
Idioma:
Zh
Año:
2015
Tipo del documento:
Article