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[Efficient genome editing in human pluripotent stem cells through CRISPR/Cas9].
Liu, Gai-gai; Li, Shuang; Wei, Yu-da; Zhang, Yong-xian; Ding, Qiu-rong.
Afiliación
  • Liu GG; Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Shanghai 200031, China.
  • Li S; Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Shanghai 200031, China.
  • Wei YD; Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Shanghai 200031, China.
  • Zhang YX; Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Shanghai 200031, China.
  • Ding QR; Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Shanghai 200031, China.
Yi Chuan ; 37(11): 1167-73, 2015 11.
Article en Zh | MEDLINE | ID: mdl-26582531
ABSTRACT
The RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has offered a new platform for genome editing with high efficiency. Here, we report the use of CRISPR/Cas9 technology to target a specific genomic region in human pluripotent stem cells. We show that CRISPR/Cas9 can be used to disrupt a gene by introducing frameshift mutations to gene coding region; to knock in specific sequences (e.g. FLAG tag DNA sequence) to targeted genomic locus via homology directed repair; to induce large genomic deletion through dual-guide multiplex. Our results demonstrate the versatile application of CRISPR/Cas9 in stem cell genome editing, which can be widely utilized for functional studies of genes or genome loci in human pluripotent stem cells.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Genoma Humano / Edición de ARN / Células Madre Pluripotentes / Sistemas CRISPR-Cas Límite: Humans Idioma: Zh Año: 2015 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Genoma Humano / Edición de ARN / Células Madre Pluripotentes / Sistemas CRISPR-Cas Límite: Humans Idioma: Zh Año: 2015 Tipo del documento: Article