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Combining Cadherin Expression with Molecular Markers Discriminates Invasiveness in Growth Hormone and Prolactin Pituitary Adenomas.
Chauvet, N; Romanò, N; Meunier, A-C; Galibert, E; Fontanaud, P; Mathieu, M-N; Osterstock, G; Osterstock, P; Baccino, E; Rigau, V; Loiseau, H; Bouillot-Eimer, S; Barlier, A; Mollard, P; Coutry, N.
Afiliación
  • Chauvet N; UMR-5203, CNRS, Institut de Génomique Fonctionnelle, Montpellier, France.
  • Romanò N; U1191, INSERM, Montpellier, France.
  • Meunier AC; UMR-5203, Université de Montpellier, Montpellier, France.
  • Galibert E; UMR-5203, CNRS, Institut de Génomique Fonctionnelle, Montpellier, France.
  • Fontanaud P; U1191, INSERM, Montpellier, France.
  • Mathieu MN; UMR-5203, Université de Montpellier, Montpellier, France.
  • Osterstock G; UMR-5203, CNRS, Institut de Génomique Fonctionnelle, Montpellier, France.
  • Osterstock P; U1191, INSERM, Montpellier, France.
  • Baccino E; UMR-5203, Université de Montpellier, Montpellier, France.
  • Rigau V; UMR-5203, CNRS, Institut de Génomique Fonctionnelle, Montpellier, France.
  • Loiseau H; U1191, INSERM, Montpellier, France.
  • Bouillot-Eimer S; UMR-5203, Université de Montpellier, Montpellier, France.
  • Barlier A; UMR-5203, CNRS, Institut de Génomique Fonctionnelle, Montpellier, France.
  • Mollard P; U1191, INSERM, Montpellier, France.
  • Coutry N; UMR-5203, Université de Montpellier, Montpellier, France.
J Neuroendocrinol ; 28(2): 12352, 2016 Feb.
Article en En | MEDLINE | ID: mdl-26686489
ABSTRACT
Although growth hormone (GH)- and prolactin (PRL)-secreting pituitary adenomas are considered benign, in many patients, tumour growth and/or invasion constitute a particular challenge. In other tumours, progression relies in part on dysfunction of intercellular adhesion mediated by the large family of cadherins. In the present study, we have explored the contribution of cadherins in GH and PRL adenoma pathogenesis, and evaluated whether this class of adherence molecules was related to tumour invasiveness. We have first established, by quantitative polymerase chain reaction and immunohistochemistry, the expression profile of classical cadherins in the normal human pituitary gland. We show that the cadherin repertoire is restricted and cell-type specific. Somatotrophs and lactotrophs express mainly E-cadherin and cadherin 18, whereas N-cadherin is present in the other endocrine cell types. This repertoire undergoes major differential modification in GH and PRL tumours E-cadherin is significantly reduced in invasive GH adenomas, and this loss is associated with a cytoplasmic relocalisation of cadherin 18 and catenins. In invasive prolactinomas, E-cadherin distribution is altered and is accompanied by a mislocalisation of cadherin 18, ß-catenin and p120 catenin. Strikingly, de novo expression of N-cadherin is present in a subset of adenomas and cells exhibit a mesenchymal phenotype exclusively in invasive tumours. Binary tree analysis, performed by combining the cadherin repertoire with the expression of a subset of known molecular markers, shows that cadherin/catenin complexes play a significant role in discrimination of tumour invasion.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Neoplasias Hipofisarias / Prolactinoma / Cadherinas / Proteínas de Unión al ARN / Galectina 3 / Adenoma Hipofisario Secretor de Hormona del Crecimiento / Securina Tipo de estudio: Prognostic_studies Límite: Adolescent / Adult / Aged / Child / Child, preschool / Female / Humans / Male / Middle aged Idioma: En Año: 2016 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Neoplasias Hipofisarias / Prolactinoma / Cadherinas / Proteínas de Unión al ARN / Galectina 3 / Adenoma Hipofisario Secretor de Hormona del Crecimiento / Securina Tipo de estudio: Prognostic_studies Límite: Adolescent / Adult / Aged / Child / Child, preschool / Female / Humans / Male / Middle aged Idioma: En Año: 2016 Tipo del documento: Article