Production of Brazilian human norovirus VLPs and comparison of purification methods.
Braz J Microbiol
; 46(4): 1265-8, 2015.
Article
en En
| MEDLINE
| ID: mdl-26691489
ABSTRACT
Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Estructurales Virales
/
Centrifugación por Gradiente de Densidad
/
Cromatografía por Intercambio Iónico
/
Virosomas
/
Norovirus
Límite:
Child
/
Humans
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Año:
2015
Tipo del documento:
Article