Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination.
Genet Mol Res
; 14(4): 18485-93, 2015 Dec 28.
Article
en En
| MEDLINE
| ID: mdl-26782496
ABSTRACT
In this study, Lactococcus lactis was engineered to express mutated internalin A on its surface and to secrete large amounts of listeriolysin O (LLO) in order to improve its potential as a vehicle for DNA vaccination. Western blotting experiments demonstrated that the bacterium expressed LLO in both the cytoplasmic and extracellular compartments, with higher quantities found in the culture supernatants. A hemolytic assay showed that the recombinant strain secreted 250 ng active LLO/mg total protein. This mInlA/LLO-producing strain of L. lactis may be used as an alternative tool in DNA vaccination against a number of infectious diseases or in cancer therapy.
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1
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
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Toxinas Bacterianas
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Proteínas Recombinantes
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Lactococcus lactis
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Proteínas de Choque Térmico
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Proteínas Hemolisinas
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Listeria monocytogenes
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Mutación
Idioma:
En
Año:
2015
Tipo del documento:
Article