Pesticide analysis using nanoceria-coated paper-based devices as a detection platform.
Analyst
; 141(5): 1837-46, 2016 Mar 07.
Article
en En
| MEDLINE
| ID: mdl-26842266
ABSTRACT
We report the first use of a paper-based device coated with nanoceria as a simple, low-cost and rapid detection platform for the analysis of organophosphate (OP) pesticides using an enzyme inhibition assay with acetylcholinesterase (AChE) and choline oxidase (ChOX). In the presence of acetylcholine, AChE and ChOX catalyze the formation of H2O2, which is detected colorimetrically by a nanoceria-coated device resulting in the formation of a yellow color. After incubation with OP pesticides, the AChE activity was inhibited, producing less H2O2, and a reduction in the yellow intensity. The assay is able to analyze OP pesticides without the use of sophisticated instruments and gives detection limits of 18 ng mL(-1) and 5.3 ng mL(-1) for methyl-paraoxon and chlorpyrifos-oxon, respectively. The developed method was successfully applied to detect methyl-paraoxon in spiked vegetables (cabbage) and a dried seafood product (dried green mussel), obtaining â¼95% recovery values for both sample types. The spiked samples were also analyzed using LC-MS/MS as a comparison to the developed method and similar values were obtained, indicating that the developed method gives accurate results and is suitable for OP analysis in real samples.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Papel
/
Plaguicidas
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Técnicas Biosensibles
/
Cerio
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Contaminantes Ambientales
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Nanopartículas
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Año:
2016
Tipo del documento:
Article