Estrogen receptor profiling and activity in cardiac myocytes.
Mol Cell Endocrinol
; 431: 62-70, 2016 08 15.
Article
en En
| MEDLINE
| ID: mdl-27164442
ABSTRACT
Estrogen signaling appears critical in the heart. However a mechanistic understanding of the role of estrogen in the cardiac myocyte is lacking. Moreover, there are multiple cell types in the heart and multiple estrogen receptor (ER) isoforms. Therefore, we studied expression, localization, transcriptional and signaling activity of ERs in isolated cardiac myocytes. We found only ERα RNA (but no ERß RNA) in cardiac myocytes using two independent methods. The vast majority of full-length ERα protein (ERα66) localizes to cardiac myocyte nuclei where it is competent to activate transcription. Alternate isoforms of ERα encoded by the same genomic locus (ERα46 and ERα36) have differential transcriptional activity in cardiac myocytes but also primarily localize to nuclei. In contrast to other reports, no ERα isoform is competent to activate MAPK or PI3K signaling in cardiac myocytes. Together these data support a role for ERα at the level of transcription in cardiac myocytes.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Miocitos Cardíacos
/
Receptor alfa de Estrógeno
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Receptor beta de Estrógeno
Límite:
Animals
/
Humans
Idioma:
En
Año:
2016
Tipo del documento:
Article