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[Role of PI3K/AKT signaling pathway in clonogenicity and tumorigenicity of CD90+ stem-like cells of the hepatocellular carcinoma cell line MHCC-97H].
Peng, Y C; Peng, N F; Xie, Z B; Lu, S D; Zhong, J H; Pang, Y B; Zhu, S L; You, X M; Li, L Q.
Afiliación
  • Peng YC; Hepatobiliary Surgery Department, Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China.
Zhonghua Gan Zang Bing Za Zhi ; 24(4): 285-90, 2016 Apr.
Article en Zh | MEDLINE | ID: mdl-27470628
ABSTRACT

OBJECTIVE:

To investigate the influence of the PI3K/AKT signaling pathway on the proportion and characteristics of the stem-like CD90(+) subpopulation of the human hepatocellular carcinoma (HCC) cell line MHCC-97.

METHODS:

MHCC-97H cultures were treated with the PI3K/AKT pathway inhibitor LY294002. The proportion of the CD90(+) subpopulation was determined by flow cytometry, and the expression of related proteins was measured by Western blot. The clonogenicity of CD90(+) and CD90(-) cells was measured by plate colony formation assay. The tumorigenicity was compared between CD90(+) and CD90(-) subpopulations (with different concentrations) in xenograft experiments in nude mice, and the changes in tumorigenicity after the addition of LY294002 were evaluated. The changes in the expression of CD90, SHP2, P-AKT, and AKT in CD90(+) and CD90(-) cell xenografts after the addition of LY294002 were examined. Data were analyzed using t test.

RESULTS:

LY294002 was capable of reducing the proportion of CD90(+) HCC stem cells from 2.98%±0.08% to 0.78%±0.08% (t = 32.400, P < 0.01) and reducing the clonogenicity of CD90(+) subpopulation from 95.13%±3.78% to 61.82%±7.23% (t = 7.617, P < 0.01). However, it showed no significant effect on the clonogenicity of CD90(-) subpopulation. LY294002 also reduced the tumorigenicity of CD90(+) subpopulation and the expression of CD90, SHP2, and P-AKT in related HCC stem cells, but it did not significantly affect the expression of AKT. LY294002 had no significant inhibitory effect on the tumorigenicity of CD90(-) cells.

CONCLUSION:

The CD90(+) subpopulation of MHCC-97H cells has the characteristics of stem cells and is dependent on the PI3K/AKT signaling pathway.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Células Madre Neoplásicas / Transducción de Señal / Carcinoma Hepatocelular / Antígenos Thy-1 / Fosfatidilinositol 3-Quinasas / Proteínas Proto-Oncogénicas c-akt Límite: Animals / Humans Idioma: Zh Año: 2016 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Células Madre Neoplásicas / Transducción de Señal / Carcinoma Hepatocelular / Antígenos Thy-1 / Fosfatidilinositol 3-Quinasas / Proteínas Proto-Oncogénicas c-akt Límite: Animals / Humans Idioma: Zh Año: 2016 Tipo del documento: Article