Combined effects of low levels of palmitate on toxicity of ZnO nanoparticles to THP-1 macrophages.
Environ Toxicol Pharmacol
; 48: 103-109, 2016 Dec.
Article
en En
| MEDLINE
| ID: mdl-27770658
ABSTRACT
We have recently proposed that the interaction between food components and nanoparticles (NPs) should be considered when evaluating the toxicity of NPs. In the present study, we used THP-1 differentiated macrophages as a model for immune cells and investigated the combined toxicity of low levels of palmitate (PA; 10 or 50µM) and ZnO NPs. The results showed that PA especially at 50µM changed the size, Zeta potential and UV-vis spectra of ZnO NPs, indicating a possible coating effect. Up to 32µg/mL ZnO NPs did not significantly affect mitochondrial activity, intracellular reactive oxygen species (ROS) or release of interleukin 6 (IL-6), but significantly impaired lysosomal function as assessed by neutral red uptake assay and acridine orange staining. The presence of 50µM PA, but not 10µM PA, further promoted the toxic effects of ZnO NPs to lysosomes but did not significantly affect other endpoints. In addition, ZnO NPs dose-dependently increased intracellular Zn ions in THP-1 macrophages, which was not significantly affected by PA. Taken together, the results of the present study showed a combined toxicity of low levels of PA and ZnO NPs especially to lysosomes in THP-1 macrophages.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Palmitatos
/
Óxido de Zinc
/
Nanopartículas
/
Macrófagos
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Año:
2016
Tipo del documento:
Article