Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly.
Cell
; 167(4): 1014-1027.e12, 2016 11 03.
Article
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| MEDLINE
| ID: mdl-27881300
ABSTRACT
Kinetochores connect centromeric nucleosomes with mitotic-spindle microtubules through conserved, cross-interacting protein subassemblies. In budding yeast, the heterotetrameric MIND complex (Mtw1, Nnf1, Nsl1, Dsn1), ortholog of the metazoan Mis12 complex, joins the centromere-proximal components, Mif2 and COMA, with the principal microtubule-binding component, the Ndc80 complex (Ndc80C). We report the crystal structure of Kluyveromyces lactis MIND and examine its partner interactions, to understand the connection from a centromeric nucleosome to a much larger microtubule. MIND resembles an elongated, asymmetric Y; two globular heads project from a coiled-coil shaft. An N-terminal extension of Dsn1 from one head regulates interactions of the other head, blocking binding of Mif2 and COMA. Dsn1 phosphorylation by Ipl1/Aurora B relieves this autoinhibition, enabling MIND to join an assembling kinetochore. A C-terminal extension of Dsn1 recruits Ndc80C to the opposite end of the shaft. The structure and properties of MIND show how it integrates phospho-regulatory inputs for kinetochore assembly and disassembly.
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MEDLINE
Asunto principal:
Kluyveromyces
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Proteínas Fúngicas
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Proteínas Cromosómicas no Histona
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Cinetocoros
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Complejos Multiproteicos
Idioma:
En
Año:
2016
Tipo del documento:
Article