Your browser doesn't support javascript.
loading
Unexpected effect of the vehicle (grain ethanol) of homeopathic FSH on the in vitro survival and development of isolated ovine preantral follicles.
Lima, Lartiza F; Rocha, Rebeca M P; Duarte, Ana Beatriz G; Brito, Ivina R; Silva, Gerlane M; Rodrigues, Giovanna Q; Nunes-Pinheiro, Diana C S; Sales, Antônia D; Moura, Arlindo A; Wheeler, Matthew B; Rodrigues, Ana Paula R; Campello, Cláudio C; Figueiredo, José Ricardo.
Afiliación
  • Lima LF; Laboratory of Manipulation of Oocytes and Preantral Follicles Department of Faculty of Veterinary, State University of Ceara, Itaperi Campus, Fortaleza, CE, 60740-903, Brazil.
  • Rocha RM; Laboratory of Manipulation of Oocytes and Preantral Follicles Department of Faculty of Veterinary, State University of Ceara, Itaperi Campus, Fortaleza, CE, 60740-903, Brazil.
  • Duarte AB; Laboratory of Manipulation of Oocytes and Preantral Follicles Department of Faculty of Veterinary, State University of Ceara, Itaperi Campus, Fortaleza, CE, 60740-903, Brazil.
  • Brito IR; Laboratory of Manipulation of Oocytes and Preantral Follicles Department of Faculty of Veterinary, State University of Ceara, Itaperi Campus, Fortaleza, CE, 60740-903, Brazil.
  • Silva GM; Laboratory of Manipulation of Oocytes and Preantral Follicles Department of Faculty of Veterinary, State University of Ceara, Itaperi Campus, Fortaleza, CE, 60740-903, Brazil.
  • Rodrigues GQ; Laboratory of Manipulation of Oocytes and Preantral Follicles Department of Faculty of Veterinary, State University of Ceara, Itaperi Campus, Fortaleza, CE, 60740-903, Brazil.
  • Nunes-Pinheiro DC; Laboratory of Manipulation of Oocytes and Preantral Follicles Department of Faculty of Veterinary, State University of Ceara, Itaperi Campus, Fortaleza, CE, 60740-903, Brazil.
  • Sales AD; Laboratory of Manipulation of Oocytes and Preantral Follicles Department of Faculty of Veterinary, State University of Ceara, Itaperi Campus, Fortaleza, CE, 60740-903, Brazil.
  • Moura AA; Group of Research in Biology of Reproduction - Department of Animal Science, Federal University of Ceará, Fortaleza, CE, Brazil.
  • Wheeler MB; Department of Animal Sciences, University of Illinois, Urbana-Champaign, Urbana, Illinois.
  • Rodrigues AP; Laboratory of Manipulation of Oocytes and Preantral Follicles Department of Faculty of Veterinary, State University of Ceara, Itaperi Campus, Fortaleza, CE, 60740-903, Brazil.
  • Campello CC; Laboratory of Manipulation of Oocytes and Preantral Follicles Department of Faculty of Veterinary, State University of Ceara, Itaperi Campus, Fortaleza, CE, 60740-903, Brazil.
  • Figueiredo JR; Laboratory of Manipulation of Oocytes and Preantral Follicles Department of Faculty of Veterinary, State University of Ceara, Itaperi Campus, Fortaleza, CE, 60740-903, Brazil.
Microsc Res Tech ; 80(4): 406-418, 2017 Apr.
Article en En | MEDLINE | ID: mdl-27921341
ABSTRACT
The aims of this study were to investigate the effects of medium replacement system (experiment I) and of FSH presentations (homeopathic - FSH 6cH and allopathic FSH - rFSH; experiment II) on the in vitro development, hormone production and gene expression of isolated ovine preantral follicles cultured for 6 days. In experiment I, secondary follicles were cultured in the α-MEM+ supplemented with FSH 6cH (0.05 fg/ml) or recombinant bovine FSH (100 ng/ml) without/with daily medium addition. The homeopathic FSH treatments with/without medium addition improved (p < .05) follicular development compared to rFSH100 treatment without addition. FSH 6cH with addition showed the highest (p < .05) estradiol production. To verify whether the effects of homeopathic FSH were not due to its vehicle, experiment II was performed. The α-MEM+ was supplemented or not with alcohol (0.2% grain ethanol, v/v), FSH 6cH or rFSH100 with daily medium addition. Surprisingly, we found that all treatments improved follicular development compared to the α-MEM+ (p < .05). Moreover, homeopathic FSH was similar to the other treatments including its vehicle. In conclusion, its vehicle (ethanol) causes the effect of homeopathic FSH on in vitro development of isolated ovine preantral follicles.
Asunto(s)
Palabras clave
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Técnicas de Cultivo de Órganos / Proliferación Celular / Etanol / Hormona Folículo Estimulante / Folículo Ovárico / Hormonas Límite: Animals Idioma: En Año: 2017 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Técnicas de Cultivo de Órganos / Proliferación Celular / Etanol / Hormona Folículo Estimulante / Folículo Ovárico / Hormonas Límite: Animals Idioma: En Año: 2017 Tipo del documento: Article