Purification of hepatitis B virus gene X product synthesized in Escherichia coli and its detection in a human hepatoblastoma cell line producing hepatitis B virus.

Chisaka, O; Araki, K; Ochiya, T; Tsurimoto, T; Hiranyawasitte-Attatippaholkun, W; Yanaihara, N; Matsubara, K

Chisaka, O; Araki, K; Ochiya, T; Tsurimoto, T; Hiranyawasitte-Attatippaholkun, W; Yanaihara, N; Matsubara, K.

Afiliación

Chisaka O; Institute for Molecular and Cellular Biology, Osaka University, Suita, Japan.

Gene ; 60(2-3): 183-9, 1987.

Article en En | MEDLINE | ID: mdl-2832251

ABSTRACT

ABSTRACT

A fused gene containing 94% of the hepatitis B virus (HBV) open reading frame X was expressed in Escherichia coli, and its 17-kDa product was purified by ion-exchange chromatography. Antibody elicited against the X-gene product reacted with materials proximal to the nuclear membrane of a human hepatoblastoma cell line producing HBV particles. No such reaction was observed with the same cell line that did not produce HBV particles.

Asunto(s)

Genes Virales; Virus de la Hepatitis B/genética; Proteínas Virales/genética; Carcinoma Hepatocelular/metabolismo; Carcinoma Hepatocelular/microbiología; Línea Celular; Clonación Molecular; Escherichia coli/genética; Escherichia coli/metabolismo; Técnica del Anticuerpo Fluorescente; Regulación de la Expresión Génica; Humanos; Neoplasias Hepáticas; Plásmidos; Proteínas Virales/biosíntesis; Proteínas Virales/inmunología

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Banco de datos: MEDLINE Asunto principal: Proteínas Virales / Virus de la Hepatitis B / Genes Virales Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Año: 1987 Tipo del documento: Article

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