DNA Release from Fe3+ -Cross-Linked Alginate Films Triggered by Logically Processed Biomolecular Signals: Integration of Biomolecular Computing and Actuation.
Chemphyschem
; 18(13): 1811-1821, 2017 Jul 05.
Article
en En
| MEDLINE
| ID: mdl-28467664
ABSTRACT
Signal-controlled release of DNA from Fe3+ -cross-linked alginate hydrogel electrochemically deposited on an electrode surface was studied. The multiple input signals were logically processed with the help of the enzyme-biocatalyzed reactions. Boolean logic gates, OR, AND, INH, were realized with the biocatalytic reactions performed by the enzymes entrapped in the alginate film. Hydrogen peroxide produced by the enzymatic reactions resulted in the degradation of the alginate hydrogel and DNA release. The alginate degradation was facilitated by the formation of free radicals in the Fenton-type reaction catalyzed by iron cations cross-linking the alginate hydrogel. The studied approach is versatile and can be adapted to various chemical signals processed by various enzymes with differently implemented Boolean logic. This work illustrates a novel concept of functional integration of biomolecular computing and actuation.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
ADN
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Compuestos Férricos
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Reactivos de Enlaces Cruzados
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Computadores Moleculares
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Alginatos
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Lógica
Límite:
Animals
Idioma:
En
Año:
2017
Tipo del documento:
Article